Toward Better Medical Diagnosis: Tissue Optical Clearing

Tissue optical immersion clearing.

In this article, we discuss the optical immersion method based on refractive index matching of scatterers (e.g., collagen, elastin fibers, cells and cell compartments) and the ground material (interstitial fluid and/or cytoplasm) of tissue and blood under the action of exogenous optical clearing agents. We analyze the optical clearing of fibrous and cell-structured tissues and blood from the po...

Recent progress in tissue optical clearing

Tissue optical clearing technique provides a prospective solution for the application of advanced optical methods in life sciences. This paper gives a review of recent developments in tissue optical clearing techniques. The physical, molecular and physiological mechanisms of tissue optical clearing are overviewed and discussed. Various methods for enhancing penetration of optical-clearing agent...

Tissue and Blood Optical Clearing for Biomedical Applications

Monte Carlo simulation on polarization features in tissue optical clearing

This paper focuses on the polarization features during the tissue optical clearing process. Based on our previous anisotropic tissue model and polarization sensitive Monte Carlo simulation program, we simulated the influence of refractive index matching on polarized photons behaviors and observed the polarization parameters change with the clearing process. Using two methods: Mueller matrix pol...

Clarifying Tissue Clearing

Biological specimens are intrinsically three dimensional; however, because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by "clearing" the tissue have been ongoing for over a century, there have been a large number of recent innovations. This Review introduces the physical basis for light scatter in tissue,...